Translational_Unit

Part:BBa_K1202111:Design

Designed by: safa tapan   Group: iGEM13_ATOMS-Turkiye   (2013-09-24)


MPG-Apoptin: Cancer killing peptide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 387
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Due to the rare codon analysis, codon optimization is performed for this part. Codons are optimized for E.coli. Additionally, a linker peptide coding region is added for the maximum correctness for our experiments. Lastly, a his tag coding region is added for the purification purposes.


Source

apoptin wss prepare from chicken anemia virus(CAV)

References